Build your workflow with PacBio Compatible

Agilent is a compatible partner for end-to-end targeted sequencing workflows including automation, library prep, and data analysis. This partnership leverages the ClearSeq Comprehensive Cancer panel and the accuracy of SBB technology to confidently identify disease-associated variants and optimize the molecular profiling of cancers. Targeted sequencing is enabled by Agilent’s SureSelect Custom DNA Target Enrichment Probes and PacBio’s sequencing by binding (SBB) technology. Additionally, Agilent’s Femto Pulse instrument is recommended for accurate generation of large-insert libraries for de novo large genome sequencing projects and other PacBio applications leveraging multi-kilobase read lengths.

Sage Science’s electrophoretic DNA size selection instruments provide user-adjustable DNA size distributions with outstanding rejection of small fragments. Optimally sized SMRTbell® libraries improve HiFi sequencing results, providing better uniformity and higher quality. PacBio recommends three systems for size selection. The BluePippin processes 4 samples with sample inputs up to 5 ug. For higher throughput needs, the PippinHT can process up to 22 samples in a single run. The SageELF (1 or 2 samples per run) fractionates an entire sample into 12 contiguous size bins, provides the most flexibility in sizing, and produces the narrowest size distributions.

The LightBench®, powered by Ranger® Technology, is a three-in-one instrument offering automated DNA size selection, fragment length analysis and fluorometric quantification. It isolates size ranges of target DNA with industry-leading recovery and precision. LightBench® excludes smaller fragments, optimizing long read inputs to deliver increased mean HiFi read lengths and data yields for PacBio sequencing platforms. To support an automated PacBio library prep workflow, the LightBench® can be integrated into 3rd party liquid handling systems thanks to its SiLA 2-compliant API.