Application: Targeted sequencing
April 24, 2024
For PureTarget, can I use samples that are not human blood and cell line extracted with Nanobind?
The only supported sample type is human blood or cell line extracted with PanDNA or CBB kits. We have been successful internally and at customer sites using other human tissues…
April 24, 2024
What guidance do you have for adding a small number of guide RNA pairs to the fixed PureTarget panel?
The PureTarget 20 gene panel is at 5 µM and includes 20 pairs of gRNAs made up of 40 individual gRNAs at 0.125 µM each. To add on custom genes…
April 24, 2024
Can I do a custom PureTarget panel?
Custom PureParget panels are not supported by PacBio Custom PureTarget panels are not officially supported by PacBio. Users must design and order their guide RNAs and optimize their custom panels…
December 18, 2023
Where can I find an overview of all HiFi applications?
Here is an overview of HiFi applications
December 14, 2023
Should I do PCR or probe-based hybridization for my targeted sequencing project?
Typically, customers with many samples find it cost-effective to design a probe panel. View the Application brief – HiFi target enrichment best practices or refer to our partnership with Twist…
December 13, 2023
Where can I find more information on specific applications?
You can find additional information on our training where you can search for and download online webinars, e-modules, and other training documents. You can also find various applications (WGS, targeted,…
December 13, 2023
What types of genomic variants can be detected from PacBio HiFi data?
PacBio HiFi reads can detect single-nucleotide variants (SNVs), small indels (<20 bp in length) and structural variants (SVs), such as insertions, deletions, duplications, inversions, translocations, short tandem repeats (STRs), and…
December 13, 2023
Can I use PacBio sequencing systems for targeted sequencing?
Yes. For general information about Targeted sequencing, please visit the targeted sequencing page, the PacBio Compatible partner targeted workflows page, or contact our technical support or field application specialists if…