We are pleased to announce the availability of our protocol for template preparation to support low DNA input for sequencing on the Sequel System. The low DNA input workflow features a 3.5-hour library prep using the SMRTbell Express Template Prep Kit 2.0 (PN 100-938-900) from as little as 150 ng of input gDNA. This workflow can be used for de novo genome assembly of up to 300 Mb and can be scaled up for larger genomes with additional gDNA input.
How low is low? This publication in the journal Genes shows how a team at the Wellcome Sanger Institute used the new method to generate a genome assembly for the Anopheles coluzzii mosquito using unamplified DNA from a single female insect. The new protocol will enable many new research areas, including performing de novo assemblies from individual small-bodied organisms.
To get started using the low DNA input workflow for your next project, we recommend you read our application note or contact your local FAS with any questions.