This blog features voices from PacBio — and our partners and colleagues — discussing the latest research, publications, and updates about HiFi sequencing.
Highly accurate long-read sequencing technologies have made it possible to generate genome assemblies at scale. In fact, HiFi sequencing is now the technology of choice for assembling reference-quality genomes…
The genomics world is buzzing. A few weeks ago, PacBio introduced SPRQ chemistry for its Revio system, a leap forward for HiFi sequencing technology that’s igniting excitement among researchers…
The first benchtop HiFi long-read sequencing platform is now here with Vega, named after one of the brightest stars in the sky. This exciting platform builds out the…
RNA sequencing (RNA-seq) has become an indispensable tool for analyzing transcriptomes across all domains of life that can reveal insights about biology and disease. While the genome remains relatively constant…
PacBio HiFi sequencing has been utilized to generate reference-quality genomes in many studies, most notably in finally completing a human genome. Following this work, the Human Pangenome Reference Consortium…
The combined accuracy and length of PacBio HiFi reads is utilized by an ever-increasing number of researchers, with numerous new publications and preprints appearing every week. Several months ago, Illumina…
Most higher organisms are diploid, meaning that each cell carries in its nucleus two copies of the individual’s genome, one from its mother and one from its father. Accurately separating…
Written by: Menzies Chen, Associate Director, Product Management, Informatics Whether it is accessing areas of the human genome that short reads can’t sequence, or powering genome assemblies across all…
The scientific community is still abuzz from last week’s announcement of the amazing achievement of the first complete sequence of a human genome by the Telomere-to-Telomere (T2T) Consortium. Assembling genomes…
What would be possible if every human genetics researcher had a complete reference genome? Today, researchers use a good – but incomplete – reference genome released in 2013. As…
Through the development of long-read, full-length RNA sequencing methods, the scientific community has recognized a fundamental paradigm shift, “from ‘gene-centric’ to ‘isoform-centric’ understanding of human transcriptomes, owing to the frequent…
Long-read PacBio HiFi sequencing has made transformative contributions to many areas of genomics over the past few years, including de novo assembly, calling of all variant types, full-length phased targeted…
These developments advance the ability of laboratories to identify and track infectious disease threats SARS-CoV-2 mutates rapidly but a new solution is now available for public health laboratories in the…
The new kid on the PacBio block — The Sequel IIe System — has been receiving high marks from universities and sequencing centers around the world. What’s it like using…
If you are like most of us at PacBio, you likely learned how to extract DNA in a high school or college biology class, or maybe even in your…
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