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December 13, 2023

Can I multiplex more than 384 amplicon samples?

Yes. With PacBio barcodes, you can create thousands of asymmetrical combinations for high-throughput multiplexing. While not all combinations have been formally validated, many customers have successfully implemented expanded multiplexing strategies.

PacBio officially supports up to 1,536-plex with the Kinnex 16S kit, and additional combinations are possible with customized barcode pairings. For guidance on setting up your project, contact your local FAS/SASS or email techsupport@pacb.com

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