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May 6, 2024

Which library prep kit should I use for human, plant, or animal WGS on HiFi sequencing systems?

24 reaction kit: SMRTbell prep kit 3.0 (102-182-700) 

96 reaction kit: HiFi prep kit 96 (103-381-200) 

Both kits and protocols use the same enzymatic steps and have qualified automated methods available. Enzyme volumes do differ between protocols/kits, and the HiFi prep kit 96 comes with additional SMRTbell cleanup beads for the automated pipette shearing method and additional fill volume to enable up to 4 automated runs of a minimum of 24 samples.  

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