HiFi difference
From eye-opening tech comparisons to inspiring examples of PacBio HiFi technology in action — this blog series explores what sets HiFi sequencing apart.
Featured article
THE HIFI DIFFERENCE – A BETTER CELL ATLAS WITH FULL-LENGTH ISOFORM SEQUENCING
Understanding the myriad of different cell types, states, and their functions is essential to understanding biology. Cell atlas projects and consortia aim to map different cell types in tissues, organs,…
The HiFi difference – immunology with a FLAIRR
Antibodies are the work horses of the adaptive immune system, identifying and neutralizing foreign objects like bacteria and viruses, and thereby are central to the science of immunology. A new…
The HiFi difference Halloween edition – DEAD GENES
Pseudogenes are typically regarded as non-functional DNA elements, and have therefore not received as much attention as their parent genes. Thanks to PacBio HiFi sequencing, many pseudogenes now RISE FROM…
The HiFi difference: Out-of-the-box 5-base sequencing
Back in April we introduced significant software enhancements to simultaneously provide the base sequence and 5-methylcytosine DNA methylation status by default, making the Sequel II/IIe systems the world’s first (and…
The HiFi difference — not being CLR
This week Illumina gave an update on their synthetic long-read approach previously named “Infinity reads,” now rebranded as Complete Long Reads (CLR). This acronym choice is of course quite ironic…
The HiFi difference – E pluribus unum
Over the past few months, we have highlighted the striking versatility of HiFi sequencing in a series of a dozen blogposts, allowing many different research applications to be performed with…
The HiFi difference – delivering superior data for proteogenomics
In living cells, it is typically the proteins that are the work horses of carrying out the myriad of biological functions, and that can be subject to aberrant processes that…
The HiFi difference: powering biodiversity genomics
In a new preprint, entitled Realizing the promise of biodiversity genomics with highly accurate long reads, a group of researchers from Utah State, BYU, the LOEWE Centre for Translational Biodiversity…
The HiFi difference: resolving the most complex genomic regions
The combined accuracy, read length, and coverage uniformity of PacBio HiFi sequence reads enables the characterization of many previously inaccessible regions in the genome. Two new preprints by Oscar Rodriguez,…
The HiFi difference: enabling the human pangenome reference
A few weeks ago, we described the use of HiFi sequencing to drive the paradigm shift from a single reference genome to a pangenome derived from a diverse set of…