PacBio Literature

This technical note describes the experimental conditions of a modified workflow preparing PacBio Kinnex single-cell libraries with Twist exome enrichment kits.

Sequencing has become an integral part of the biopharma research and development process and enhances our understanding of the molecular determinants of health and disease. Whether you are conducting research on novel potential drug targets, biomarkers, or therapeutics such as gene therapies, PacBio® offers highly accurate sequencing solutions across its portfolio that can improve your chances of success and may reduce your time to market.

This technical note describes the procedure for targeted enrichment using IDT xGen Hyb and Wash kit v3 for KinnexTM full-length RNA libraries.

With HiFi target enrichment you can easily and cost-effectively sequence just the genes you care about at scale. Design custom gene panels with our partners at Twist, sequence them with long and accurate HiFi reads, and get all the benefits you expect from HiFi reads: comprehensive detection of single nucleotide variants, structural variants, and indels. HiFi target enrichment is the only technology that can resolve complex gene families like HLA or Cytochrome P450 genes with haplotype resolution.​

PacBio® HiFi sequencing enables greater variant detection in more regions of the genome than alternative technologies, providing exceptional insight at any depth. Choosing the right level of sequencing coverage is a critical first step for project planning

Highly accurate long reads – HiFi reads – with single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly.

Microbial genomics with PacBio long-read HiFi sequencing is accurate, scalable, and cost effective. Automated end-to-end workflows from extraction to analysis and optimized kits drive greater throughput for HiFi microbial sequencing at lower cost.

This technical note demonstrates the HiFi sequencing performance of saliva samples collected and stabilized with DNA Genotek Oragene devices and extracted using PacBio Nanobind kits.

SMRT® Link software tools for PacBio® HiFi sequencing systems are designed to set up and monitor sequencing runs, review performance metrics, and analyze, visualize, and annotate sequencing data. SMRT Link is available for both local installation and cloud access, offering flexibility to work in different environments depending on your lab’s needs.

Application brief — What can you do with one SMRT Cell

Powered by the exceptional accuracy of HiFi sequencing and the Tandem Repeat Genotyping Tool (TRGT), the PacBio® PureTarget™ repeat expansion panel offers more comprehensive genotyping for 20 of the most important repeat expansions for human health. This application note demonstrates the performance of the PureTarget repeat expansion panel, and presents the PureTarget panel as a scalable, more comprehensive solution for profiling repeat expansions, compared to legacy genotyping and next-generation sequencing methods.

Reveal the complexity of the human genome to power human disease research — With the advancement of highly accurate long-read sequencing, missing pieces could be resolved and fully complete the genome. PacBio HiFi sequencing gave rise to this achievement and has dramatically improved understanding of the human genome by resolving gaps that are unattainable by other sequencing methods, accurately mapping complex regions, and enabling more contiguous genome assemblies.

Vega benchtop system brochure

PureTarget offers an easy and scalable workflow to capture repeat expansions and brings you from sample to answer in three days. Compatible with the RevioTM and Sequel® II systems, PureTarget libraries can be multiplexed with up to 48 samples, generating deep coverage across the 20-gene target panel.

Vega system specification sheet