PacBio Literature

PacBio® HiFi sequencing enables greater variant detection in more regions of the genome than alternative technologies, providing exceptional insight at any depth. Choosing the right level of sequencing coverage is a critical first step for project planning

Highly accurate long reads – HiFi reads – with single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly.

Microbial genomics with PacBio long-read HiFi sequencing is accurate, scalable, and cost effective. Automated end-to-end workflows from extraction to analysis and optimized kits drive greater throughput for HiFi microbial sequencing at lower cost.

This technical note demonstrates the HiFi sequencing performance of saliva samples collected and stabilized with DNA Genotek Oragene devices and extracted using PacBio Nanobind kits.

SMRT® Link software tools for PacBio® HiFi sequencing systems are designed to set up and monitor sequencing runs, review performance metrics, and analyze, visualize, and annotate sequencing data. SMRT Link is available for both local installation and cloud access, offering flexibility to work in different environments depending on your lab’s needs.

Application brief — What can you do with one SMRT Cell

Powered by the exceptional accuracy of HiFi sequencing and the Tandem Repeat Genotyping Tool (TRGT), the PacBio® PureTarget™ repeat expansion panel offers more comprehensive genotyping for 20 of the most important repeat expansions for human health. This application note demonstrates the performance of the PureTarget repeat expansion panel, and presents the PureTarget panel as a scalable, more comprehensive solution for profiling repeat expansions, compared to legacy genotyping and next-generation sequencing methods.

Reveal the complexity of the human genome to power human disease research — With the advancement of highly accurate long-read sequencing, missing pieces could be resolved and fully complete the genome. PacBio HiFi sequencing gave rise to this achievement and has dramatically improved understanding of the human genome by resolving gaps that are unattainable by other sequencing methods, accurately mapping complex regions, and enabling more contiguous genome assemblies.

Vega benchtop system brochure

PureTarget offers an easy and scalable workflow to capture repeat expansions and brings you from sample to answer in three days. Compatible with the RevioTM and Sequel® II systems, PureTarget libraries can be multiplexed with up to 48 samples, generating deep coverage across the 20-gene target panel.

Vega system specification sheet

See it all with HiFi sequencing on the Revio™ system

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

This application note details a wide array of bioinformatics tools for full length- isoform sequencing across isoform classification and annotation, fusion transcript discovery, variant calling, transcript assembly, differential transcript analysis, differential splicing usage, and transcript visualization.

This technical note describes the experimental conditions of a modified workflow for preparing PacBio® Kinnex single-cell libraries with single-cell or single-nucleus cDNA generated using Parse Biosciences Evercode Whole Transcriptome products, the WT and WT Mini kits. This workflow has not been tested using the Evercode WT Mega kit.