Sequencing 101
Follow along to learn more about our technology, reveal the impacts of long-read sequencing, and uncover tips on how to get started.
Featured article
Sequencing 101: long-read sequencing
Long-read sequencing technologies are quickly becoming the new gold standard in genomics research, and HiFi sequencing is at the forefront. This article provides an introductory look at what long-read sequencing is, and explores topics the advantages, applications of using long-read sequencing in your research.
Sequencing 101: DNA extraction — tips, kits, and protocols
If you are like most of us at PacBio, you likely learned how to extract DNA in a high school or college biology class, or maybe even in your…
Genomes vs. genNNNes: the difference between contigs and scaffolds in genome assemblies
Updated on February 24, 2023. Originally published September, 2016. In recent interactions with the scientific community, we’ve seen a growing number of questions around scaffolding genome assemblies. We thought…
Sequencing 101: ploidy, haplotypes, and phasing — how to get more from your sequencing data
Geneticists often point out that a human does not have “a” genome but rather two genomes: one inherited from the mother and another from the father. The number of…
Sequencing 101: what’s the value of sequencing full-length RNA transcripts?
The study of genomics has revolutionized our understanding of science, but the field of transcriptomics grew with the need to explore the functional impacts of genetic variation. While different tissues…
Sequencing 101: from DNA to discovery — the steps of SMRT sequencing
Starting a sequencing project can be daunting. First of all, there are several types of sequencing technologies, each based on unique processes. At PacBio, we use a technology called Single…
Sequencing 101: understanding accuracy in DNA sequencing
For scientists who utilize DNA sequencing in their research but are not experts in the underlying technology, it can be difficult to determine the accuracy of sequencing results — and…
Sequencing 101: looking beyond the single reference genome to a pangenome for every species
What is a pangenome? Unless you have an identical twin, no other person has a genome that is identical to yours. The same is true for other animal, plant, and…
Sequencing 101: why are long reads important for studying viral genomes?
The COVID-19 pandemic has brought a sudden urgency to virus research and led many of us to dig more deeply into all the tools available for characterizing viral genomes, from…
Sequencing 101: video introduction to PacBio sequencing and the Sequel II system
We’re pleased to release a short video describing PacBio sequencing and our latest platform, the Sequel II system. If you’ve ever wondered how Single Molecule, Real-Time (SMRT) sequencing works, what…