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Plant + animal genomics

Empowering plant and animal science with precision: Unlocking actionable genomic insights through advanced long-read sequencing to tackle global challenges

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Plant + animal genomics solutions

In the face of a changing climate and growing populations, highly accurate long-read sequencing technology gives plant and animal scientists access to actionable genomic insights that other technologies cannot provide.

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Agrigenomics

Accelerate breeding programs and increase resilience to pests, disease, and climate challenges.

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Biodiversity

Explore the biodiversity of populations, species and ecosystems to build a more detailed view of the biome.

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Plant + animal biology

Address questions about evolution, development, gene expression, transcription regulation, and more.

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Transforming plant + animal research with full-length and single-cell isoform sequencing

In this whitepaper, you’ll discover how full-length and single-cell isoform sequencing with the Iso-Seq method can enhance your plant and animal research. Learn how combining Iso-Seq with Kinnex kits boosts throughput, making PacBio long reads a cost-effective RNA sequencing solution for your projects.

Plant + animal genomics in action

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Genome wide detection and phasing of genetic and epigenetic variants from a single library prep

Simultaneously measure methylation by detecting 5mC at CpG sites.

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Building haplotype-resolved pangenome databases for improved marker development

With third-generation sequencing tools, researchers have been able to better impute complex traits…

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INCREASING THE THROUGHPUT OF FULL-LENGTH 16S AND RNA-SEQ WITH KINNEX KITS

Discover the transformative power of Kinnex kits for advancing plant and animal research.

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Did you know we have a library of articles, reports, papers, and videos related to plant + animal genomics?

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Publication

A pangenome and pantranscriptome of hexaploid oat

Oat grain is a traditional human food rich in dietary fiber that contributes to improved human health. Interest in the crop has surged in recent years owing to its use as the basis for plant-based milk analogs. Oat is an allohexaploid with a large, repeat-rich genome that was shaped by subgenome exchanges over evolutionary timescales. In contrast to many other cereal species, genomic research in oat is still at an early stage, and surveys of structural genome diversity and gene expression variability are scarce.

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Blog

Lighting a SPRQ: How new Revio chemistry fuels innovative research

Join us as we dive into the details of SPRQ chemistry, how it compares to the first iteration of Revio technology, and what it means for the future of genomics. SPRQ (pronounced “spark”) chemistry is enabled by a new sequencing polymerase and optimized reagents that ensure more DNA molecules reach the surface of a SMRT Cell during the loading process. This optimization kickstarts improved efficiency throughout the entire sequencing workflow. With increased output, coupled with reduced time, DNA input, and cost, SPRQ chemistry changes the game for HiFi sequencing.

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Advantages of using HiFi sequencing for your plant + animal genomics research

 

Highly accurate genomic sequencing helps uncover genomic clues from the organisms that populate our world. Complex genomes and transcriptomes provide pathways to protect crop and livestock health and better understand plant and animal biology at a molecular level.
 

Here are some advantages of HiFi sequencing to support your plant and animal research:

  • Build more complete, accurate, and phased reference genomes for all types of organisms
  • Confidently call variants at every scale, including SNPs, structural variants, copy number variants, translocations, and homopolymer repeats
  • Uncover new biology with Kinnex RNA kits that provides full-length, phased transcript sequence — profile isoforms and capture 5’ untranslated regions
  • Reveal previously hidden regulatory mechanisms with epigenetic sequence information

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Figure 1: Changes in land plant genome assembly quality and availability over time. Marks, R.A., Hotaling, S., Frandsen, P.B. et al. Representation and participation across 20 years of plant genome sequencing. Nat. Plants 7, 1571–1578 (2021). https://doi.org/10.1038/s41477-021-01031-8

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Applications to fuel plant + animal genomics research

Whole genome sequencing

Assemble haplotype-resolved, more complete, and accurate de novo genomes and simultaneously detect methylation in the face of challenging complexity, such as high ploidy, high GC content, and highly repetitive sequences.

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Structural variant calling

Resolve variants at every scale — from SNPs and INDELs to larger structural variants like copy number variations and translocations, even in complicated regions of the genome.

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Targeted sequencing

Accurately detect and uncover variant types even in hard-to-reach regions of the genome to power genotyping studies, breeding programs, and more.

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RNA sequencing

Uncover novel biology with full-length transcripts at scale with the Kinnex RNA kits. Profile isoforms and capture variants in 5’ UTRs for the data you need to build more accurate genome annotations.

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Plant + animal genomics applications with PacBio HiFi systems

Application Samples per Vega SMRT Cell Samples per Revio SMRT Cell
Whole genome sequencing
De novo assembly
(2 Gbs genome)
1 2
Variant detection
(2 Gbs genome)
Structural varients: 3
All variants: 1
Structural varients: 6
All variants: 2
Microbial de novo assembly
(2 Gb total)
384 384
RNA sequencing
Kinnex single-cell RNA sequencing 1 (3,000-6,000 cells) 1 (6,000-10,000 cells)
Kinnex full-length RNA sequencing
5M reads 6 12
10M reads 3 6
Targeted sequencing
Amplicon sequencing >1,000 >1,000
Microbial sequencing
Shotgun metagenomic profiling 64 communities 128 communities
Shotgun metagenomic assembly 8 communities 16 communities
Kinnex 16S rRNA 1,024 communities 1,536 communities
All sample throughputs are estimates for either the Vega system with 1 SMRT Cell or the Revio system using SPRQ chemistry with 1 SMRT Cell. Coverage may vary based on sample quality, library quality, and fragment lengths. Currently available SMRTbell® adapter index plates 96A-96D contain a total of 384 SMRTbell barcoded adapters. Microbial de novo assembly assumes microbes with 2 Gb of total genome size at 30x per sample. Single-cell transcriptomics assumes ≥80 million reads per library on the Revio system and ~50-60 million reads per library on the Vega system. Full-length RNA sequencing assumes a total of 60M reads for Revio SPRQ and 30M reads for Vega, regardless of plexity. Amplicon sequencing assumes a 12-hour movie time for 1–5 kb, 24-hour movie time for 5+ kb, and >50× per sample. Target enrichment assumes >50× per sample.

HiFi sequencing price and throughput on the Revio and Vega systems

Vega system Revio with SPRQ chemistry
$1,100 USD per SMRT Cell for sequencing1 $995 USD per SMRT Cell for sequencing1
One sequencing stage that generate 60 Gb per run Four independent sequencing stages that generate 120 Gb per SMRT Cell for multi-application runs
Simultaneous epigenetic information from native DNA sequencing applications Simultaneous epigenetic information from native DNA sequencing applications

1 US list price is $1,100 for sequencing reagents for one Vega SMRT Cell, which has an expected yield of 60 Gb.

1 US list price is $995 for sequencing reagents for one Revio SMRT Cell, which has an expected yield of 120 Gb.

Long-read sequencing helps answer biological questions across a broad range of plant + animal sciences

Break through the barriers of polyploidy and heterozygosity

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Insect biology

Develop disease prevention methods and improve pest control

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Crop improvements

Improve crop health, conduct trait selection, and enhance breeding efficiency

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Animal health + breeding

Study patterns of inheritance, immunology and domestication

plant + animal genomics

Environmental health

Identify the role of microbes in complex communities and characterize and monitor pathogens.

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Marine biodiversity

Explore ecosystems to study evolution and address the challenges of climate change

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Conservation

Examine genomic diversity of species-at-risk for assisted reproduction, wildlife management, and sustainability

Long-read value for RNA applications

Application Full-length RNA Single-cell RNA 16S rRNA
Sequencing goal Isoform discovery and abundance information Cell type-specific isoform isoform discovery and abundance information Species identification in metagenomics samples
Short-read shortcomings Incorrect isoform discovery and abundance information No isoforms. Only genes No species identification
Other long-read drawbacks

Lower accuracy poor transcript identification and variant calling

Lower accuracy poor transcript identification and variant calling

Lower accuracy poor transcript identification and variant calling

HiFi advantages Isoform discovery and abundance information Cell type-specific isoform discovery Species-level identification
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THIS IS YOUR MOMENT

Long reads at a lower cost and higher throughput. Highly accurate short reads. From the fullest picture to the finest detail, your moment of discovery is waiting.

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