Clavibacter michiganensis subsp. michiganensis (Cmm) is a gram positive actinomycete, causing bacterial canker of tomato (Solanum lycopersicum) a disease that can cause significant losses in tomato production. In this study, we determined the complete genome sequence of 13 California Cmm strains and one saprophytic Clavibacter strain using a combination of Ilumina and PacBio sequencing. The California Cmm strains have genome size (3.2 -3.3 mb) similar to the reference strain NCPPB382 (3.3 mb) with =98% sequence identity. Cmm strains from California share =92% genes (8-10% are noble genes) with the reference Cmm strain NCPPB382. Despite this similarity, we detected significant alternatives in California strains with respect to plasmid number, plasmid composition, and genomic island presence indicating acquisition of unique mechanisms controlling virulence. Plasmids pCM1 and pCM2, that were previously demonstrated to be required for NCPPB382 virulence, also differ in their presence and gene content across Cmm strains. pCM2 is absent in some Cmm strains and that still retain virulence in tomato. Saprophytic Clavibacter possess a novel plasmid, pSCM, and lacks the majority of characterized virulence factors. Genome sequence information was also used to design specific and sensitive primer pairs for Cmm detection. A mechanistic understanding of how genomic changes have impacted Cmm virulence and survival across diverse strains will be necessary for developing a robust disease control strategies for bacterial canker of tomato.
Organization: University of California, Davis
Year: 2015