Nanobind + SRE sample prep kits

Nanobind DNA extraction

Higher DNA quality means better HiFi data

Nanobind extracted DNA is optimized for HiFi sequencing. Our kits provide you with an all-in-one extraction experience that can be used with a wide range of sample types. The PanDNA kit supports manual high-quality extraction from cultured cells, bacteria, whole blood, saliva, tissue, plant nuclei, and insect samples in 1 to 2.5 hours. The Nanobind HT CBB kit enables automation of DNA extraction for human and animal blood, mammalian cells, and bacteria. Our HT kit is compatible with up to four robotic platforms and 96 samples that can be extracted in 2.5 hours.

Pan DNA icons for sample types

Nanobind PanDNA HMW DNA extraction protocols

Select the appropriate Nanobind PanDNA Procedure & checklist to use, based on sample type and starting material

Sample type	Starting material	Samples input	Workflow time	Procedure & checklist
Blood	Human whole blood	200 µL	~1 hr.	Extracting HMW DNA from human whole blood using Nanobind kits (102-573-500)
		Nucleated red blood cells (nRBCs)	2.5 - 20 µL	~1 hr.	Extracting HMW DNA from nucleated red blood cells (102-574-000)
		Human whole blood with RBC lysis	400 µL	<1.5 hrs.	Extracting HMW DNA from human whole blood with RBC lysis (103-377-500)
Saliva	Human saliva collected in DNA Genotek™ Oragene™ devices	500 µL	~2 hrs.	Extracting HMW DNA from saliva collected in DNA Genotek™ Oragene™ devices (103-544-000)
Animal tissue	Diverse tissue types	2 - 100 mg	~2.5 hrs.	Extracting HMW DNA from animal tissue (102-574-600)
Insect tissue	Insect whole body or segment	>20 mg	~2.5 hrs.	Extracting HMW DNA extraction from insects (102-377-400)
Plant tissue	Isolated plant nuclei	0.25 - 5g	~1.5 hrs.¹	Extracting HMW DNA from plant nuclei (103-378-200)
Mammalian cultured cells	Suspension cell culture	1 x 10⁶ – 5 x 10⁶ diploid human cells	~1 hr.	Extracting HMW DNA from cultured suspension cells (103-394-500)
Mammalian cultured cells		Adherent cell culture	1 x 10⁶ – 5 x 10⁶ diploid human cells	~1 hr.	Extracting HMW DNA from cultured adherent cells (102-573-600)
Cultured bacteria	Gram-negative bacteria	5 x 10⁸ – 5 x 10⁹ bacterial cells	~1 hr.	Extracting HMW DNA from Gram-negative bacteria (102-573-800)
Cultured bacteria		Gram-positive bacteria	5 x 10⁸ – 5 x 10⁹ bacterial cells	~1 hr.	Extracting HMW DNA from Gram-positive bacteria (102-573-900)
1Upstream plant nuclei prep isolation procedure (e.g., 102-574-900 or 102-574-800) typically take ~3 hours to complete.

See example of result in PanDNA kit brochure and detail on the kit in PanDNA kit guide and overview

How does it work?

Nanobind kits contain specific reagents to process and lysate diverse sample types. Following lysis, the DNA binds to Nanobind disks which shield bound DNA from damage during extraction, resulting in HMW DNA. A final Short Read Eliminator (SRE) size selection step helps remove DNA fragments below 10 kb.

larger image of Automated workflow for sample prep image showing lysis, binding, washing, elution, library prep, sequencing and analysis

Brochure

Automate DNA extraction and optimize your sample prep workflow for HiFi sequencing

PacBio Nanobind HT kits enable automation of DNA extraction, the first step of an end-to-end workflow that harnesses the power of HiFi sequencing. Nanobind HT kits are designed for whole human blood and mammalian cell samples and are compatible with four robotic platforms.

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Roundel image for cover of Nanobind HT brochure

SHORT READ ELIMINATOR (SRE) KITS

Simple size selection for optimal HiFi result

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SRE treatment following DNA extraction helps removing DNA molecules below 10 kB. It results in improved HiFi read length distribution and HiFi sequencing yield. The SRE workflow is fast, easy, and inexpensive using simple size selective precipitation. HMW DNA is precipitated by centrifugation and low molecular weight (LMW) DNA is washed away. Many samples can be process in parallel in 2 hours (10 min hands-on time). HT solution will be available soon.

Procedure + checklist

Removing short DNA fragments with the Short Read Eliminator (SRE) kit

This procedure describes the workflow for DNA size selection to remove molecules <10 kb using the SRE kit on HMW DNA before shearing and library preparation. The SRE kit is used for rapid size selection of unsheared HMW DNA samples. This method can significantly enhance mean read length by progressively depleting short DNA up to 10 kb.

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