Library prep + barcoding

The HiFi prep kit 96 is composed of:

• 1 – HiFi prep kit 96 (103-122-600)
• 1 – Elution buffer (101-633-500)
• 1 – SMRTbell adapter index plate 96A (102-009-200)
• 1 – SMRTbell cleanup beads-85 mL (103-294-600)
• 1 – Buffer LTE HT (103-306-100)

 
Supports 96 samples per kit.

Compatible with the Revio system, Vega system, and Sequel II and IIe systems.

• Resource: Procedure & checklist - Preparing whole genome libraries using the HiFi prep kit 96
• Resource: Guide & overview - Automated HiFi prep 96 and HiFi annealing binding and cleanup for the Hamilton NGS Microlab STAR system
• Resource: Guide & overview - Short Read Eliminator SRE DNA shearing and cleanup for the Hamilton Microlab Prep system
• Product insert: HiFi prep kit 96
• Product insert: Elution buffer
• Product insert: SMRTbell adapter index plate 96A
• Product insert: SMRTbell cleanup beads-85mL
• Product insert: Buffer LTE HT
• SDS: HiFi prep kit 96
• SDS: Elution buffer
• SDS: SMRTbell adapter index plate 96A
• SDS: SMRTbell cleanup beads-85mL
• SDS: Buffer LTE HT

The HiFi plex prep kit 96 is composed of:

• 1 – HiFi plex prep kit 96 (103-122-800)
• 1 – Elution buffer (101-633-500)
• 1 – SMRTbell cleanup beads-52 mL (103-294-500)
• 1 – Buffer LTE HT (103-306-100)

 
Supports 96 samples per kit.

Compatible with the Revio system, Vega system, and Sequel II and IIe systems.

• Resource: Procedure & checklist - Preparing multiplexed whole genome and amplicon libraries using the HiFi plex prep kit 96
• Resource: Guide & overview - Automated HiFi plex prep 96 for the Hamilton NGS Microlab STAR system
• Resource: Guide & overview - Short Read Eliminator SRE DNA shearing and cleanup for the Hamilton Microlab Prep system
• Product insert: HiFi plex prep kit 96
• Product insert: Elution buffer
• Product insert: SMRTbell cleanup beads-52mL
• Product insert: Buffer LTE HT
• SDS: HiFi plex prep kit 96
• SDS: Elution buffer
• SDS: SMRTbell cleanup beads-52mL
• SDS: Buffer LTE HT

Now available for pre-order with shipment commencing Dec 2024!

The Revio SPRQ HiFi prep kit 96 is composed of:

• 1 – AMPure PB (100-265-900)
• 1 – Elution buffer (101-633-500)
• 1 – SMRTbell adapter index plate 96A (102-009-200)
• 1 – HiFi prep kit 96 (103-122-600)
• 1 – SRE HT kit (103-124-500)
• 1 – Revio SPRQ polymerase kit 96 (103-497-000)
• 1 – SMRTbell cleanup beads-85 mL (103-294-600)

 
Supports 96 samples per kit.

Compatible with the Revio system.

• Product insert: AMpure PB
• Product insert: Elution buffer - 50 mL
• Product insert: SMRTBell adapter index plate 96A
• Product insert: HiFi prep kit 96
• Product insert: SRE HT kit
• Product insert: Revio SPRQ polymerase kit 96
• Product insert: SMRTbell cleanup beads - 85 mL
• SDS: AMpure PB
• SDS: Elution buffer
• SDS: SMRTBell adapter index plate 96A
• SDS: HiFi prep kit 96
• SDS: SRE HT kit
• SDS: Revio SPRQ polymerase kit 96
• SDS: SMRTbell cleanup beads - 85 mL

The SMRTbell cleanup bead kit is comprised of:

• 1 – SMRTbell cleanup beads (102-158-300)
• 1 – Low TE buffer (pH 8.0) (102-178-400)

Supports 24 samples per kit.

Compatible with the Revio system, Vega system, and Sequel II and IIe systems.

• Product insert: Low TE buffer
• Product insert: SMRTbell cleanup beads
• SDS: Low TE buffer
• SDS: SMRTbell cleanup beads

The SMRTbell prep kit 3.0 is comprised of:

• 1 – SMRTbell prep kit 3.0 (102-141-700)
• 1 – SMRTbell cleanup beads (102-158-300)
• 1 – Low TE buffer (pH 8.0) (102-178-400)

 
Barcoded adapters and size-selection reagents are sold separately.

Supports 24 SMRTbell libraries per kit.

Compatible with the Revio system, Vega system, and Sequel II and IIe systems.

• Resource: Procedure & Checklist — Preparing whole genome and metagenome libraries using SMRTbell® prep kit 3.0
• Resource: Procedure & Checklist — Preparing multiplexed amplicon libraries using SMRTBell prep kit 3.0
• Resource: Guide & overview — Short Read Eliminator SRE DNA shearing and cleanup for the Hamilton Microlab Prep system
• Product insert: SMRTbell prep kit 3.0
• Product insert: SMRTbell cleanup beads
• Product insert: Low TE buffer (pH 8.0)
• SDS: SMRTbell prep kit 3.0
• SDS: SMRTbell cleanup beads
• SDS: Low TE buffer (pH 8.0)

The AMPure PB beads size selection kit is composed of:

• 1 – AMPure PB (100-265-900)
• 1 – Elution buffer (101-633-500)

 
Reagent quantity supplied at 6 mL volume.

Compatible with the Sequel system, Sequel II and IIe systems, and Revio system.

• Resource: Procedure & Checklist — Preparing whole genome and metagenome libraries using SMRTbell® prep kit 3.0
• Product insert: AMPure PB beads
• Product insert: Elution buffer
• SDS: AMPure PB beads
• SDS: Elution buffer

The HiFi express template prep kit 2.0 is composed of:

• 1 – SMRTbell express template prep kit 2.0 (100-938-900)
• 1 – SMRTbell enzyme cleanup kit 2.0 (101-932-600)
• 1 – Sequencing primer v5 (102-067-400)

 
Supports 18 reactions per kit.

Compatible with the Sequel II and IIe systems.

• Resource: Procedure & Checklist — Preparing HiFi SMRTbell Libraries Using SMRTbell Express Template Prep Kit 2.0
• Resource: Procedure & Checklist — Using AMPure PB Bead for Size Selection
• Product insert: SMRTbell express template prep kit 2.0
• Product insert: SMRTbell enzyme cleanup kit 2.0
• Product insert: Sequencing primer v5
• SDS: SMRTbell express template prep kit 2.0
• SDS: SMRTbell enzyme cleanup kit 2.0
• SDS: Sequencing primer v5

The components of the Onso fragmentation DNA library prep kit are packaged together in a single box.

The Onso fragmentation library prep kit enables simultaneous fragmentation and end repair using a low-bias enzymatic fragmentation/end repair formulation to process genomic DNA samples. The kit supports processing of 5–500 ng of high-molecular gDNA input.

Supports processing of up to 96 samples per kit.

Compatible with the Onso system.

• Product insert: Onso fragmentation DNA library prep kit
• SDS: Onso fragmentation DNA library prep kit

The components of the Onso DNA library prep kit are packaged together in a single box.

The Onso library prep kit enables fragmented DNA inputs (e.g., enzymatic, mechanically-sheared, or native) to undergo single-step end-repair and A-tailing reactions to prepare for ligation to adapters.

Suitable sample types supported include cell-free DNA, PCR amplicons, and any pre-fragmented DNA at 10-1000 ng input range.

Supports 96 samples per kit.

Compatible with the Onso system.

• Product insert: Onso DNA library prep kit
• SDS: Onso DNA library prep kit

The components of the Onso library amp kit are packaged together in a single box.

The resulting amplicons can be used for library QC, as input to downstream applications (e.g., target enrichment), and as input to the Onso clustering/sequencing process.

Supports 96 reactions per kit.

Compatible with the Onso system.

• Product insert: Onso library amp kit
• SDS: Onso library amp kit

The components of the Onso library quant kit are packaged together in a single box.

The Onso library quant kit enables accurate quantification of Onso-compatible libraries by qPCR. The quant kit contains six standards ranging from 100pM to 0.001pM and includes qPCR master mix, Onso-specific PCR primers, dilution buffer, and ROX dyes (hi/lo) for use across different qPCR instruments.

Supports 500 reactions per kit.

Compatible with the Onso system.

• Product insert: Onso library quant kit
• SDS: Onso library quant kit

The components of the Onso library conversion kit are packaged together in a single box.

The kit contains Onso-specific primers and 2X PCR master mix optimized to maintain library fidelity during the conversion process.

Supports 96 reactions per kit.

Compatible with the Onso system.

• Product insert: Onso library conversion kit
• SDS: Onso library conversion kit

The components of the Onso indexed library control kit are packaged together in a single box.

The kit is provided as an indexed library at 2nM concentration, ready to dilute and spike-in to samples of interest prior to clustering and sequencing.

Supports up to 50 sequencing runs per kit.

Compatible with the Onso system.

• Product insert: Onso indexed library control
• SDS: Onso indexed library control

The components of the Onso blocker oligos are packaged together in a single box.

The Onso blocker oligos are designed to be utilized in hybridization capture-based workflows in place of traditional P5/P7 blocking oligos. With optimized design specific to Onso indexed adapters, it decreases the frequency of non-specific binding of adapter sequences during the hybridization reaction.

Supports 96 reactions per tube.

Compatible with the Onso system.

• Product insert: Onso blocker oligos
• SDS: Onso blocker oligos