The Complete PacBio Workflow
We have introduced novel technologies at every stage of the sequencing process — from preparation to data analysis — to ensure an efficient workflow that delivers exceptional, precise results.
Sample prep
- High molecular weight DNA extraction for HiFi sequencing
- Validated protocols for extraction of a diverse set of sample types
Library preparation
- Accepts a variety of sample types and insert sizes
- Size-selection options to enrich for longest inserts
- Multiplexing and barcoding solutions available
HiFi Sequencing
- Flexible sequencing times
- Process up to 8 SMRT Cells 8M in one run
- Data available for analysis as each SMRT Cell completes
- Fully automated: No manual touch points during a sequencing run
Analytical software
- Variety of analysis methods available through SMRT Analysis and PacBio bioconda
- Open source software
- Advanced data visualization and mining
Workflow
EMPOWER YOUR SINGLE-CELL TRANSCRIPTOME STUDY WITH MAS-SEQ
- 16-fold throughput increase using MAS-Seq for 10x Single Cell 3’ kit
- From cDNA to sequencing-ready library in 2 days
- Compatible with Sequel II/IIe and Revio systems
- SMRT Link workflow outputs gene- and isoform- matrix compatible with tertiary software
Workflow
ADVANTAGES OF HIGHLY ACCURATE HIFI READS FOR VERIFYING AAV CONSTRUCTS
PacBio’s AAV end-to-end workflow and flexible protocol allow scientists to comprehensively profile packaged genomes as individual intact molecules to identify potential contaminants that can affect the safety, efficacy, and cost-effectiveness of AAV vector gene therapy.
- Discover novel potential capsid constructs
- Conduct vector quality control experiments
- Assess the safety of resulting constructs
Whole genome sequencing
For humans, plants, animals and microbes including de novo sequencing and variant detection
RNA sequencing
In-depth analysis of cDNA sequences across the entire transcriptome or targeted genes
Epigenetics
Detect DNA modifications in your samples while you sequence on the PacBio platform
