Expedited assessment of terrestrial arthropod diversity by coupling Malaise traps with DNA barcoding 1.

Authors: deWaard, Jeremy R and Levesque-Beaudin, Valerie and deWaard, Stephanie L and Ivanova, Natalia V and McKeown, Jaclyn T A and Miskie, Renee and Naik, Suresh and Perez, Kate H J and Ratnasingham, Sujeevan and Sobel, Crystal N and Sones, Jayme E and Steinke, Claudia and Telfer, Angela C and Young, Andrew D and Young, Monica R and Zakharov, Evgeny V and Hebert, Paul D N

Monitoring changes in terrestrial arthropod communities over space and time requires a dramatic increase in the speed and accuracy of processing samples that cannot be achieved with morphological approaches. The combination of DNA barcoding and Malaise traps allows expedited, comprehensive inventories of species abundance whose cost will rapidly decline as high-throughput sequencing technologies advance. Aside from detailing protocols from specimen sorting to data release, this paper describes their use in a survey of arthropod diversity in a national park that examined 21?194 specimens representing 2255 species. These protocols can support arthropod monitoring programs at regional, national, and continental scales.

Journal: Genome
DOI: 10.1139/gen-2018-0093
Year: 2019

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