Figure 4. The Nighthawk workflow. Nighthawk builds a colored De Bruijn graph from read overlaps. Overlaps are scored by shared variants between two reads. Overlaps with negative RSS indicate cross-phase overlaps and are removed. The resulting overlaps are passed to a string graph assembler (such as FALCON) for phased assembly. Figure 5.

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Early access users have praised the new protocol for its ease, output and speed. “Never got this much data and long polymerase read length,” noted one customer. “cDNA length is also way better than any previous data.” The new Iso-Seq Express also supports multiplexing up to 12 samples for additional savings.

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Start with the recommended input of high-quality DNA (50–300 ng)1,2. Shear DNA to ~10–15 kb with low- or high-throughput methods for flexible batch sizes to obtain fragment lengths optimal for HiFi sequencing3. Use the HiFi plex prep kit 961 to multiplex up to 384 samples, or up to 375 Mb or 1.2 Gb of total genome per SMRT® Cell 8M or 25M ...

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Our revolutionary sequencing technologies combine the completeness of long reads with the accuracy of short reads to provide the most comprehensive view of genomes, transcriptomes, and epigenomes. Unlock the potential of PacBio sequencing using our portfolio of tools to analyze, visualize and annotate your sequencing data.

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Page 5 Experimental Protocol STEP Preparing RiboMinus Magnetic Beads Notes 1 Resuspend the RiboMinus Magnetic Beads in its bottle by thoroughly vortexing. 2 For each sample, pipet 250 μL of the bead suspension into a sterile, RNase-free, 1.5 mL LoBind tube. 3 Place the tube/s with the bead suspension on a magnetic rack for 1 minute. Gently aspirate and discard the supernatant.

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Create high-quality whole genome de novo assemblies of eukaryotic organisms. Call all variant types with the highest precision and recall for small and large variants. Survey large population cohorts and resolve structural variants. Read full-length transcripts to characterize isoform diversity. The Sequel II system, powered by Single Molecule ...

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User Bulletin. This Bulletin describes PacBio's best practices for thawing the Sequel Sequencing Kit 2.1. It is highly recommended to follow this procedure to completely dissolve any solids which may be present in the wells of the deep well plate. The 8-reaction Sequel Sequencing Kit 2.1 includes two tubes of OS Enzyme to ensure compatibility ...

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Improve your HiFi WGS sequencing workflow with the Femto Pulse system. Getting the most out of your long-read whole genome sequencing (WGS) workflow requires accurately characterizing the size distribution of your genomic DNA and SMRTbell® libraries. The Agilent Femto Pulse system is the recommended quality control (QC) technology for PacBio ...

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Refer to third-party user guide documenation Qubit 4 fluorometer system Femto Pulse system Guide & overview –Nanobind PanDNA kit (103-394-800) Nanobind PanDNA kit (103 -260 000 / 24 RXN) n Procedure & checklist –Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0 (102-166-600) SPK 3.0 protocol reference

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Nanobind kits use a standard lyse, bind, wash, elute workflow. For automated Nanobind HT extraction kits, each plate (or plate row in the case of the KingFisher Duo Prime instrument) contains a different buffer or sample solution. The Nanobind disk is transferred from one plate to another and mixed. Magnetic rod.

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