More than 150 SMRT Sequencing users gathered at Stanford University for our annual West Coast User Meeting & workshops earlier this month. Many thanks to all the scientists who attended...

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Watch on. Although each sequencing project is unique, there are five main steps to go from DNA to discovery with SMRT sequencing: Step 1: sample prep. Similar to cooking, for the best results, start with the best ingredients. The ideal sequencing starter is high molecular weight DNA. There are plenty of kits on the market that can help with ...

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the protocol on the DreamPrep NGS Compact, interactive user prompts guide the user through the options and explain how to load the deck (Figure 2). Figure 2: View of the touchscreen with TouchTools™ user prompts for definition of the run options. The protocol includes three independent scripts: 1) an initial SMRTbell clean-up bead purification

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Refer to the Safety Data Sheet (SDS) for information on reagent hazards and protocols for safe handling, use, storage, and disposal. Multiplexing best practices Multiplexing can be achieved with one of the three following methods: 1. Barcoded cDNA primers using Iso-Seq primers bc01–12 in step 3 of the protocol. To multiplex, use the Iso-Seq

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In this PacBio User Group Meeting presentation, Jonas Korlach and Roberto Lleras share the latest updates to the structural variation application and. Menu. Products. Sequencing systems. Revio long-read system; Onso short-read system; Sequel long-read systems; Technologies. HiFi long-read sequencing;

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Refer to protocols for additional recommendations for reagent handling. Shelf life Refer to expiration date on kit label. Intended use Research use only. Not for use in diagnostic procedures. Support1 For additional product and technical information, such as safety data sheets (SDS) or procedures, visit our website. Legal

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Remaining substitution errors in these highly-filtered reads are likely dominated by mis-incorporations during amplification. Therefore, we compared the impact of several commercially-available high-fidelity PCR kits with full-length 16S amplification. We show results of our experiments and describe an optimized protocol for full-length 16S ...

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SMRTbell Library Preparation and Sequencing (PN 101-599-700) PacBio protocol document describes methods for targeted PCR amplification and construction of a multiplexed library using SMRTbell Express Template Prep Kit 2.0 for sequencing full-length 16S genes on the Sequel, Sequel II and Sequel IIe Systems (Sequel Systems). This document ...

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In this step, incorporate programmable Kinnex segmentation adapter sequences into amplified DNA products. 1 16S full-length sequence capture. Set up 12 parallel Kinnex PCR reactions per 16S sample with premixed Kinnex primers to generate amplified DNA products containing programmable sequences at both ends. Safe stop.

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User Group Meeting: FALCON-Phase: Phased diploid assemblies through integration of PacBio and Hi-C data In this PacBio User Group Meeting presentation, Zev Kronenberg of PacBio presents on using the combination of PacBio and Phase Genomics data and analysis tools to create highly contiguous genome assemblies.

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