Refer to protocols for additional recommendations for reagent handling. Shelf life Refer to expiration date on kit label. Intended use Research use only. Not for use in diagnostic procedures. Support For additional product and technical information, such as safety data sheets (SDS) or procedures, visit our website. Legal

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protocols for use with different automated sample ... plate filling and limited user interaction during the run Nanobind HT kits enable automated high-throughput HMW DNA extraction for PacBio HiFi sequencing Nanobind HT kits are designed for human/animal blood, mammalian cells, and bacteria, and are compatible ...

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Two PacBio users shared their experiences in the May 7 webinar, “Understanding SARS-CoV-2 and host immune response to COVID-19 with PacBio sequencing.” ... These include two microbial sequencing protocols — the Mt. Sinai 1.5 kb and 2 kb amplicon protocol and the Eden 2.5 kb protocol — as well as guidelines for target-based capture using ...

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No complicated algorithms for barcode correction or orthogonal sequencing data are required. With the Kinnex single-cell RNA kit, you can: Achieve 16-fold throughput increase on Sequel II/IIe and Revio systems. Move from gene counting to full-length isoform information. Reveal cell type-specific spliced isoforms and expressed variants.

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If plant tissues are larger than ~0.5 cm2, place 1-4 g of frozen tissue on a clean, chilled surface, and quickly mince to ≤0.5 cm2 pieces using a scalpel. Transfer minced tissue to the empty 50 mL conical tube (from step 1). Add 10 mL of ice-cold Buffer NIB to the minced tissue. Keep the tube on ice during the entire disruption process.

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New Consumables-SMRTbell Enzyme Clean Up Kit 2.0 (101-932-600) (NEW)-Sequencing Primer v5 (102-067-400) (NEW)-Polymerase Binding Kit 2.2 (101-894-200) (NEW)Updated HiFi Sample Prep Protocol for De Novo Assembly and Variant Detection-Enables reduced minimum input gDNA (≥5 µg) for running multiple SMRT Cells-Supports high-throughput sample processing and automation

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Here, we present DNA extraction protocols for seven marine species (four invertebrates, two algae, and a marine yeast), optimized to provide sufficient DNA quality and yield for de novo genome sequencing projects. Journal: Methods in molecular biology. DOI: 10.1007/978-1-4939-3774-5_2. Year: 2016.

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some plant species, LN2 grinding may result in improved DNA size. It is recommended that users start with the TissueRuptor protocol. Either fresh or frozen plant material can be used. Up to 5 g of plant material can be input into the LN2 protocol and up to 4 g of plant material can be put into the TissueRuptor protocol.

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Substitution errors in these highly filtered reads may be dominated by mis-incorporations during amplification. Sequence differences in full-length 16S amplicons from several commercial high-fidelity PCR. were compared. We show results of our experiments and describe our optimized protocol for full-length 16S amplification for SMRT Sequencing.

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Template Prep Kit 2.0 (PN 101-730-400) protocol document describes how to prepare SMRTbell libraries with low DNA input amounts for sequencing on the Sequel, Sequel II and Sequel IIe Systems for WGS de novo assembly applications using HiFi reads. The procedure describes preparing HiFi libraries from >150 ng of input genomic DNA (gDNA) for the ...

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