USER MANUAL: g-TUBEU. TUBE INTRODUCTIONThe g-TUBE is a single use consumable designed to shear genomic DNA into large fragments, with a mean ranging from 6 kbp to 20 kbp. g-TUBE works with a bench top centrifuge and the final size of the DNA fragments is controlled by the acceleration rate and spee.

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PacBio HiFi sequencing has exceptionally accurate long reads and unbiased coverage required to provide comprehensive whole genome sequencing.

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Finally, Shana McDevitt from the California Institute for Quantitative Biosciences shared the core lab perspective as she discussed sample size, purity requirements, and extraction protocols for PacBio sequencing.

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This protocol describes the extraction of HMW DNA from 5–30 μL of nucleated blood. It has been validated on fish, bird, and lizard blood including fresh, frozen, and ethanol preserved blood samples. This protocol requires the Nanobind CBB kit (102-301-900) or Nanobind tissue kit (102-302-100).

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24 rxn. Product PN: 102-208-300. orage and handling requirementsProduct should be sto. ed ambient between 15 to 30 oC.Refer to protocols for additional recom. or reagent handling.Shelf lifeRefer. date on kit label.

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This procedure describes the workflow for constructing whole genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell®prep kit 3.0 for sequencing on PacBio®long-read systems. This procedure may be performed manually or using one of the many qualified automation methods for SMRTbell prep kit 3.0. Overview Libraries per SMRTbell prep kit 3.0 1–24 QC and workflow ...

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With increased computing power and advanced on-instrument data processing, the new Sequel IIe System can directly produce HiFi reads, and save users time and money in the process. Learn more about the latest evolution of our award-winning long-read sequencing technology.

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This protocol describes the extraction of HMW DNA from animal tissue using Qiagen TissueRuptor II for sample homogenization. This protocol has been validated for many mammalian, avian, and fish tissue types including heart, liver, spleen, kidney, and colon. This protocol requires the Nanobind tissue kit (102-302-100).

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Why HiFi sequencing is the best choice for microbiome and metagenomics research. What makes the new Kinnex 16S rRNA kit and Revio system a game-changer for microbiology. Details on new downloadable HiFi microbiome and metagenomics datasets.

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Learn how to perform DNA extraction for optional results including what kits and protocols work well for highly accurate long-read sequencing.

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