In this PacBio User Group Meeting presentation, PacBio scientist Meredith Ashby shared several examples of analysis — from full-length 16S sequencing to shotgun sequencing — showing how SMRT Sequencing enables accurate representation for metagenomics and microbiome characterization, in some cases even without fully assembling genomes. New updates will provide users with a dedicated ...

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This protocol describes the extraction of HMW DNA from cultured cells. This protocol has been validated on several cell types including GM12878 and MCF-7. This protocol requires the Nanobind CBB kit (102-301-900) or Nanobind tissue kit (102-302-100).

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As PacBio customers are upgrading to the new PacBio® RS II System, some of our core lab users have already begun blogging about the improved results. At the University of...

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Kinnex 16S + Revio take the best in microbiome sequencing and make it better. More data, more value, more insights. Building on the trusted performance of the Sequel II generation, PacBio has changed the game again in microbial ecology and metagenomics with the release of the Revio system. The massive 15x throughput increase on this powerful ...

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Iso-Seq was developed by Pacific Biosciences (PacBio), and it is capable of identifying new isoforms with extraordinary precision due to its long-read technology. The technique to create libraries is straightforward, and the PacBio RS II instrument generates the information in hours.

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Learn how to perform DNA extraction for optional results including what kits and protocols work well for highly accurate long-read sequencing.

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The table below shows recommended Blue Pippin run setup protocols for size selection. For the latest BluePippin User Manual and guidance on size-selection protocols, please contact Sage Science

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Tissue disruption with TissueRuptor is faster and typically results in higher extraction yields than LN2 grinding. For some plant species, LN2 grinding may result in improved DNA size. It is recommended that users start with the TissueRuptor protocol (Procedure & checklist – Isolating nuclei from plant tissue using TissueRuptor disruption).

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This User Bulletin describes PacBio's best practices for loading recommendations and a reminder on the required use of the Sequel® Internal Control. It is highly recommended that you read this before loading your sample on the Sequel System.

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December 12, 2023 | Products, procedures + protocols. Kinnex launch promises to revolutionize RNA research. RNA sequencing (RNA-seq) has become an indispensable tool for analyzing transcriptomes across all domains of life that can reveal insights about biology and disease. While the genome remains relatively constant for most species over brief ...

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