Wenger offered a look at updated structural variant calling protocols for the Sequel System, including how to tune parameters such as sequence coverage, and shared quick case studies. If you missed his CoLab talk, you can check out the ASHG poster he presented today or watch a similar presentation he gave at our User Group Meeting this summer.

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Press the pipette tip to the center of the well gently until the seal breaks. Touch the bottom of the well with the tip. Do not attempt to open the foil completely to the edge of the well by following the perimeter of the well with the tip. 2. Discard the tip and add the sample to the respective wells. 3.

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There is so much to discover with HiFi reads. Beyond SMRT Link, which offers a graphic interface to execute fully-developed analysis workflows, PacBio research is constantly innovating with new tools that uncover more from HiFi sequencing.. This page highlights some of the popular tools and workflows for HiFi applications. To explore the complete list of tools developed by PacBio visit the ...

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The Agilent Femto Pulse system automated pulsed-field CE instrument is a fast, high-resolution benchtop capillary electrophoresis (CE) platform that utilizes pulsed-field electrophoresis to separate high molecular weight DNA fragments. This platform allows important DNA quality checkpoints to be completed in less than 1.5 hours with minimal sample input for de novo large genome sequencing ...

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Prepare and Run the Gel. The following procedure is for the Standard Casting Stand 14 cm x 13 cm frame for use with any BIO-RAD CHEF System. Prepare 3 liters of 0.5X TBE Electrophoresis Buffer. In a 1 Liter graduated cylinder, add 50 mL of 10X TBE Electrophoresis Buffer to 950 mL of deionized water. Repeat two more times for a total of 3 liters.

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This reference guide describes recommended procedures for sequencing HLA Class I and Class II genes for producing fully-phased, unambiguous, allele-level information on the Sequel®, Sequel II and Sequel IIe Systems (Sequel Systems). HLA genes can be amplified using commercial assays (e.g., GenDx) or customers’ own custom assays.

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both ends of the cDNA share a common sequence: 5’ AAG CAG TGG TAT CAA CGC AGA GTA C 3’ However, the primer included in the Clontech kit may not be sufficient for the Iso-Seq procedure. We recom-mend purchasing additional primers from any oligo synthesis vendor (e.g., IDT, Operon, etc.). Prepare a stock of

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Highly accurate long reads, known as HiFi reads, are a new tool in scientists’ sequencing toolbox. Hear PacBio users share how they are using HiFi reads to explore the genomes, transcriptomes, metagenomes, and the benefits HiFi reads provide for their addressing critical life science questions. HiFi reads Help Scientists Explore Genomes and ...

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High-throughput Plant + Microbial Sequencing Workflows. These protocols are designed for high-throughput (HT) data generation of plant and microbial genomes on PacBio long-read sequencing systems. These end-to-end workflows were developed with a reputable industry partner and enable extraction through library preparation at scale for thousands ...

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Note that library preparation of varying genomic DNA will require user optimization for the fragmentation time and size selection steps. Recommendations made in this protocol are starting guidelines. Thermal cycler program Program thermal cycler(s) prior to beginning the protocol for the first time. 1. Fragmentation, end-repair and A-tailing

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