The SMRTbell Express Template Prep Kit 2.0 provides a streamlined, single-tube reaction strategy to generate SMRTbell libraries from 500 bp to >50 kb insert size targets to support large-insert genomic libraries, multiplexed microbial genomes and amplicon sequencing. With this new formulation, we have increased both the yield and efficiency of SMRTbell library preparation for SMRT Sequencing ...

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Hamilton’s and PacBio’s collective goal is to provide a walk-away automation solution for HMW DNA extraction, to make sample preparation easier for their customers, and to facilitate the upscaling of important downstream applications in genomics and in the healthcare setting. “Sample preparation is often overlooked and underappreciated.

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Today offered something new for the PacBio® team: our SMRT® technology was the subject of a tweet chat hosted by Genome Biology. For one hour, editors at the journal along...

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Along the way, we will share guidelines and our latest best practices for data collection and high quality genome assembly that will be of use to the PacBio user community. 10:00 – 10:25 a.m. ... User Group Meeting Agenda – September 7, 2017 Hosted by the Puglisi Laboratory Stanford University School of Medicine—Paul Berg Hall.

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This Application note introduces the PacBio WGS Variant Pipeline that consolidates several state-of-the-art analysis tools into a single user-friendly workflow. This piece also serves as a best practice guide for streamlining the analysis of HiFi data to generate compelling biological insight. 2023 PacBio.

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We have assembled an experienced team of technical support scientists, engineers, and bioformaticians to assist you with all of your support needs. Email: [email protected]. Monday–Friday. 7am–5pm (PST) 1.877.920.PACB (7222) Customer login to open a new support case or review case history.

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This document applies to Sequel® and Sequel II Systems using SMRT® Link v9.0. Note: For information on SMRT Link v8.0, see the document SMRT Analysis Barcoding Overview v8.0, available here. SMRT Link v9.0 includes a streamlined end-to-end barcoding workflow for automated analysis of multiplexed samples. It enables users to define barcodes ...

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R gene enrichment and Sequencing (RenSeq, Jupe et al. 2013) is a genome complexity reduction method which allows to enrich for nucleotide-binding, leucine reach repeat (NLR) type plant disease resistance genes prior to sequencing. RenSeq was established and successfully used with Illumina platforms (Jupe et al. 2013, Andolfo et al. 2014), however the repetitive nature of NLR genes hampered de ...

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Since the launch of the Iso-Seq protocol in SMRT Analysis in 2014, the analysis pipeline has seen several improvements, as well a host of new tools. ... For more detailed information on cDNA sequencing with PacBio, don’t miss this primer on GitHub and the shared protocol on SampleNet. [1] Pan et al. (2008) Deep surveying of alternative ...

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with universal sequences are designed and supplied by users. In the second-round of PCR, barcodes are incorporated by using universal sequences tailed with 16 bp PacBio barcode sequences. A ready-to-use reagent kit containing 96 Barcoded Universal Primers (BUP) in a plate format is commercially available from Pacific Biosciences.

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