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AGBT 2020 Highlights: Reference-Grade Assemblies, Iso-Seq Data, and ...

She also reported on a new pangenome reference project, which aims to include sequence data from 350 individuals and generate telomere-to-telomere assemblies. Watch Graves-Lindsay’s full AGBT 2020 presentation: Generating High Quality Human Reference AssembliesTop of Form. Generating High Quality Human Reference Assemblies with PacBio Sequencing.


Sequel IIe system latest system release - PacBio

SMRT Link 11.0 is now available for all Sequel II and Sequel IIe system users. Key improvements include support for our new consumables and library prep protocols, new real-time, detailed run status monitoring for all associated instruments, and support for all of our new sequencing workflows including 5-base HiFi sequencing.


Nanobind PanDNA kit - pacb.com

The all-new PacBio® Nanobind® PanDNA kit consolidates the capabilities of our existing sample-specific offerings into. single solution for DNA extraction. This kit provides users with an all-in-one extraction experience that can be used with. wide range of sample types. The PanDNA kit supports high-quality extraction from cultured cells ...


HiFi sequencing and software v11.0 release: Technical overview for ...

• New whole genome sequencing (WGS) library prep protocol using SPK 3.0 enables reduced workflow times and reduced DNA input requirements; and eliminates gel-based size selection requirements for constructing large/small genome WGS and shotgun metagenomic libraries • Amplicon, Iso-Seq method, and HiFiViral SARS-CoV-2 library prep protocols ...


A comprehensive model of DNA fragmentation for the ... - PacBio

During DNA extraction the DNA molecule undergoes physical and chemical shearing, causing the DNA to fragment into shorter and shorter pieces. Under common laboratory conditions this fragmentation yields DNA fragments of 5-35 kilobases (kb) in length. This fragment length is more than sufficient for DNA sequencing using short-read technologies which generate reads 50-600 bp in length, but ...


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