Elevate your research with highly accurate full-length RNA information made available by the PacBio Kinnex kits. Kinnex offers full-length isoform discovery and abundance information, cell type-specific isoform discovery, and species-level metagenomics community identification that are difficult to capture with short reads.

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This protocol was developed using the Hamilton NGS STAR MOA system. Please refer to the Guide & overview for HiFi plex prep kit 96 for step-by-step instructions for running this protocol on the NGS STAR MOA system. The HiFi plex prep kit 96 contains enough fill volume to support up to 4 x 24 sample automated runs. If setting up

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PacBio SBB sequencing is a Q40+ short-read sequencing technology that works by measuring the light signals from fluorescently labeled nucleotides when they are bound –but not incorporated– by a polymerase on a DNA strand. A computer then matches these light signals with a corresponding nucleotide base call. Unlike other short-read ...

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The PacBio Sequel II and IIe systems provide all scientists with access to high throughput, cost effective, highly accurate long-read sequencing. Watch this short video to learn how the Sequel II and IIe systems can help you be confident in your discoveries made possible with comprehensive views of genomes, transcriptomes, and epigenomes.

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The new workflow dramatically reduces the requirements for DNA quantity. Now, scientists need only 5 ng of genomic DNA to kick off a SMRT Sequencing project — that’s less than 2% of the starting volume needed for our current low DNA input protocol. This opens up access to HiFi sequencing for researchers studying the tiny arthropod species ...

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Kohli et al., Androgen Receptor Variant AR-V9 Is Coexpressed with AR-V7 in Prostate Cancer Metastases and Predicts Abiraterone Resistance, Clinical Cancer Research (2017) Iso-Seq data identified AR-V9 often co-expressed with AR-V7. Iso-Seq data re-annotated the cryptic exons CE3 and CE5 as a single 3’ exon with different splice sites.

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Follow the 10x Chromium user guide up until cDNA amplification, cleanup and QC (refer to 10x Chromium user guide for 5’ kits and 3’ kits). Before you begin, evaluate the quantity and size distribution of input cDNA to determine whether it is suitable for the protocol (average size between 500– 1500 bp). a Step Instructions

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using the HTTPS protocol. If an SSL certificate is not installed with SMRT Link, the application will use the PacBio self-signed SSL certificate and will use the HTTP protocol. In this case, each user will need to accept the browser security warnings described in “Using the PacBio® self-signed SSL certificate” on page 120 .

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The protocols for these methods are designed to be intuitive and accessible through integrated touchscreens with TouchTools™, ensuring ease of use. ... library preparation protocols in DreamPrep NGS solutions will enable users to upscale their genomic projects faster. Tecan Group Ltd. makes every effort to include accurate and up-to-date ...

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This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio® Sequel® II, Sequel IIe, and RevioTM systems. Barcoded 16S-specific primers (12 forward and 32 reverse) can be used in different combinations allowing for the multiplexing of up to 384 samples on one SMRT® Cell.

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