Iso-Seq express 2.0 kit Product PN: 103-071-500. ess 2.0 kitProduct PN: 103-071-500DescriptionThe Iso-Seq express 2.0 kit contains reagents and oligos. for generat. ng full-length cDNA (24 reactions). It includes12 unique barcoded cDNA primers for multiplexing and is c.

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To perform this procedure, you must have reviewed the User Bulletin - Guidelines for Preparing cDNA Libraries for Isoform Sequencing (Iso-Seq™). Below are the three available procedures for specific project requirements. This procedure described in this document is for isoform sequencing without size selection. Procedure Size Selection Target ...

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This procedure provides instructions for using AMPure PB beads for size-selection of SMRTbell libraries. AMPure PB beads are diluted with PacBio Elution Buffer to 35% (volume by volume) and then used for size-selection. Important: To efficiently remove SMRTbell templates <3 kb or <5 kb, be sure to use this procedure after.

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Onso fragmentation DNA library prep kit PN: 102-499-100. The Onso fragmentation DNA library prep kit is for the creation of Onso-compatible libraries from unfragmented, HMW DNA. The kit includes reagents for fragmentation, end-repair, A-tailing, and adapter ligation of DNA fragments. Purchase.

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Kinnex single-cell RNA library preparation procedure supports up to 4-plex sample multiplexing through use of 4 different barcoded Kinnex SMRTbell adapters2. PacBio Documentation (103-254-300) Kinnex full-length RNA library prep protocol uses Kinnex single-cell RNA kit. Do not use SMRTbell prep kit 3.0 with this protocol.

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Title: Product brochure - Sequel IIe system - Sequencing evolved Author: PacBio Subject: Learn how highly accurate long-read sequencing from the Sequel IIe system delivers data you can trust for advanced biological insights across a range of applications.

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This protocol requires at least 15 ng of 10x Chromium 3’ single cell cDNA. Follow the 10x Chromium user guide up until cDNA amplification, cleanup and QC (refer to Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 user guide). Before you begin, evaluate the quantity and size distribution of input cDNA to determine whether it is suitable for

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More than 150 SMRT Sequencing users gathered at Stanford University for our annual West Coast User Meeting & workshops earlier this month. Many thanks to all the scientists who attended...

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HiFi sequencing is a single-molecule, long-read sequencing technology that produces reads that are both long and accurate. HiFi sequencing was developed by PacBio and is the core chemistry run on all PacBio long-read sequencing instruments. HiFi sequencing has its origins in the nanofluidic designs and single molecule real-time chemistry ...

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Pulsed-field electrophoresis system separates large DNA samples by size, and then fractionates the whole sample into 12 fractions. One sample is fractionated on a single precast agarose cassette, and 1 or 2 cassettes may be processed at one time. Min. / Max. Load: 100 ng / 5 g (sheared gDNA) per lane.

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