This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. See Other Documentation for product inserts, safety data sheets (SDS) and more.

---

Use of these protocols is offered to those customers who understand and accept the associated terms and conditions and wish to take advantage of their potential to help prepare samples for analysis using the PacBio® System.

---

Use of these protocols is offered to those customers who understand and accept the associated terms and conditions and wish to take advantage of their potential to help prepare samples for analysis using the PacBio® system.

---

This guide describes an optimized procedure for preparing your DNA template for sequencing on the PacBio System. This Blunt-End Liga-tion protocol, is suitable for all libraries (greater than 250 bp amplicons) and offers a total preparation time of approximately four hours. SMRTbellTM Templates.

---

Guidelines for Using PacBio® Barcodes for SMRT® Sequencing . A set of 384 barcodes, each comprised of 16 bp, are custom-designed for the PacBio System. By adding these barcodes to PCR primers, users can perform parallel or multiplex sequencing using SMRT Analysis v1.4 or later.

---

Shared Protocol - Extracting DNA using Phenol-Chloroform. Please note: the shared protocols described herein may not have been validated by Pacific Biosciences and are provided as-is and without any warranty.

---

This Bulletin provides recommendations and tips for preparing 20 kb SMRTbell templates using the BluePippinTM size-selection method using size-selection cutoffs between 6 kb and 10 kb. Once you have read this User Bulletin, proceed to the Procedure & Checklist - 20 kb Template Preparation and Sequencing for pre-paring your 20 kb SMRTbell templates.

---

Guidelines for Preparing 20 kb SMRTbell™ Templates. This procedure can be used to prepare size-selected libraries from 5 μg of sheared and concentrated DNA using the BluePippin Size-Selection system.

---

This SMRTbell library cleanup protocol involves a) binding annealed PacBio DNA SMRTbell libraries to magnetic beads, b) washing the bound annealed DNA SMRTbell templates to remove potential contaminants, and c) eluting the purified, annealed DNA SMRTbell templates from the magnetic beads.

---

a user-friendly interface that guides selection & alignment & organizes work into projects for intuitive navigation Install on the platform of your choice: Windows®, Linux ®, or Macintosh Easily add users to collaborate, share protocols, & data within your institution which saves time & leverages your collective knowledge Schedule tasks to ...

---