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Explore these publicly available datasets generated with PacBio sequencing.

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Featured datasets

Wastewater analysis on the Onso system

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Kinnex full-length RNA with heart sample

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PureTarget repeat expansion panel with Coriell samples

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Whole genome datasets     Targeted datasets     RNA datasets     Metagenomic datasets    AAV datasets  COVID datasets    File formats

Whole genome sequencing

Application Dataset Download literature Technology Sequencing system
Variant detection, assembly, epigenetics Homo sapiens - GIAB trio HG002-4 Application brief HiFi long read Revio system - SPRQ chemistry
Tumor/normal COLO829 melanoma Application note HiFi long read Revio system - SPRQ chemistry
Whole genome sequencing - Fiber-Seq chromatin assay Homo sapiens - HG002 Blog HiFi long read Revio system - SPRQ chemistry
Variant detection, assembly, epigenetics Homo sapiens — GIAB trio HG002-4 Application note HiFi long read Revio system
Tumor/normal COLO829 melanoma Application note HiFi long read Revio system
Tumor/normal HCC1395 Application note HiFi long read Revio system
Whole genome sequencing Various plant & animals – mouse, ladybug, oak, mistletoe, and maize Plant + animal biology HiFi long read Revio system
Whole genome sequencing Homo sapiens - GIAB trio HG002-4 Application brief SBB short read Onso system
Assembly (ultra-low DNA input) Phlebotomus papatasi, Homo sapiens, Drosophila melanogaster Considerations for using ultra-low DNA input workflows for WGS HiFi long read Sequel II system
Assembly Food safety & infectious microbes – 96 plex Microbial WGS HiFi long read Sequel IIe system

Targeted sequencing

Application Dataset Download literature Technology Sequencing system
PureTarget Repeat expansion panel Coriell samples PureTarget brochure HiFi long read Sequel IIe system
PureTarget Repeat expansion panel RFC1 samples PureTarget brochure HiFi long read Sequel IIe system
PureTarget Repeat expansion panel HG001 and HG002 48-plex PureTarget brochure HiFi long read Revio system
PureTarget Repeat expansion panel HG001 and HG002 8-plex PureTarget brochure HiFi long read Sequel IIe system
Target enrichment IDT xGen cfDNA & FFPE dataset Technical note SBB short read Onso system
Target enrichment SeraCare ctDNA w/Agilent capture panel SBB sequencing on SeraCare ctDNA using Agilent hybrid capture panel SBB short read Onso system
Target enrichment Twist Exome 2.0 NA12878 8-plex Application brief - germline variant detection on Onso SBB short read Onso system
Target enrichment Twist Alliance Dark Genes Panel HiFi sequencing with Twist Bioscience target enrichment HiFi long read Revio system
Target enrichment Twist Alliance Dark Genes Panel Twist Alliance Dark Genes Panel HiFi long read Sequel IIe system
Target enrichment Twist Alliance Long Read PGx Panel Twist Alliance Dark Genes panel HiFi long read Sequel IIe system
Target enrichment Twist Custom BRCA1/2 Panel HiFi sequencing with Twist Bioscience target enrichment HiFi long read Sequel IIe system
Amplicon sequencing CYP2D6 amplicon HiFi amplicon sequencing for pharmacogenetics: CYP2D6 HiFi long read Sequel IIe system

RNA sequencing

Application Dataset Download literature Technology Sequencing system
Onso single-cell Homo sapiens - PBMC 10x Chromium Single Cell 3' (1k and 10k cells) N/A SBB short-read Onso system
Kinnex single-cell Homo sapiens - PBMC 10x Chromium Single Cell (5' and 3' libraries) Kinnex single-cell application note HiFi long-read Sequel II and Revio systems
Kinnex single-cell Homo sapiens - HG002 (10x 5') Kinnex single-cell RNA application note HiFi long-read Revio system
Kinnex full-length RNA Homo sapiens - UHRR Kinnex full-length RNA application note HiFi long-read Revio system - SPRQ chemistry
Kinnex full-length RNA Homo sapiens – UHRR Kinnex full-length RNA application note HiFi long-read Sequel II and Revio systems
Kinnex full-length RNA Homo sapiens – HG002 Kinnex full-length RNA application note HiFi long-read Revio system
Kinnex full-length RNA Homo sapiens - Heart Kinnex full-length RNA application note HiFi long-read Revio system
Kinnex full-length RNA Homo sapiens - Cerebellum Kinnex full-length RNA application note HiFi long-read Revio system

Metagenomics

Application Dataset Download literature Technology Sequencing system
Kinnex 16S rRNA ZymoBIOMICS Fecal Reference with TruMatrix Technology (human) Kinnex full-length 16S rRNA application note HiFi long read Sequel II and Revio systems
Kinnex 16S rRNA Mixture: ZymoBIOMICS Gut Microbiome Standard, ZymoBIOMICS Fecal Reference with TruMatrix™ Technology, ATCC 20 Strain Even Mix Genomic Material, ATCC 20 Strain Staggered Mix Genomic Material Kinnex full-length 16S rRNA application note HiFi long read Sequel II and Revio systems
Metagenomic profiling and assembly ZymoBIOMICS Fecal Reference with TruMatrix Technology (human) Microbiome and metagenome sequencing with HiFi reads HiFi long read Revio system
Metagenomic profiling and assembly Zymo wastewater analysis Zymo wastewater surveillance application note SBB short read Onso system
Metagenomic profiling and assembly ZymoBIOMICS Fecal Reference with TruMatrix Technology (human) Microbiome and metagenome sequencing with HiFi reads HiFi long read Sequel IIe system
Metagenomic profiling and assembly 20 strain mock microbial community – ATCC MSA-1003 – shotgun Microbiome and metagenome sequencing with HiFi reads HiFi long read Sequel II system
Metagenomic profiling and assembly Human gut microbiome pooled standards Microbiome and metagenome sequencing with HiFi reads HiFi long read Sequel IIe system
Full-length 16S sequencing 20 strain mock microbial community – ATCC MSA-1003 – 16S Microbiome and metagenome sequencing with HiFi reads HiFi long read Sequel II system

Gene therapy vectors

Application Dataset Download literature Technology Sequencing system
AAV scAAV 8-plex – Vector Biolabs rh10-CBA-eGFP AAV sequencing with HiFi reads HiFi long read Sequel II system
AAV ssAAV – ViGene pAV-CMF-GFP AAV sequencing with HiFi reads HiFi long read Sequel II system
AAV ssAAV-scAAV 1:10 mixture AAV sequencing with HiFi reads HiFi long read Sequel II system
AAV ssAAV - Charles River - pAV-CMF-GFP AAV sequencing with HiFi reads HiFi long read Revio system

COVID-19 sequencing

Application Dataset Data type PacBio system
SARS-CoV-2 surveillance Omicron Samples HiFi reads Sequel II system
SARS-CoV-2 surveillance Synthetic RNA controls HiFi reads Sequel II system
SARS-CoV-2 surveillance Surveillance samples HiFi reads Sequel II system

File formats

Dataset Data type PacBio system
8 SMRT Cell run directory Directory Revio system
reads.bam and hifi_reads.bam HiFi reads Sequel IIe system
reads.bam and hifi_reads.bam with kinetic information HiFi reads Sequel IIe system

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