Support FAQs

No. PacBio does not accept samples for sequencing. See the list of our global certified service providers (CSPs) by geographic region.

Email support@pacb.com with the name of your employer or institution and your address and we will connect you to the relevant team for your organization.

You can find additional information on our training where you can search for and download online webinars, e-modules, and other training documents. You can also find various applications (WGS, targeted, epigenetics, RNA and metagenomics) on our sequencing methods page. If you have any questions about our training materials, contact us.

Here is an overview of HiFi applications

The SPRQ (pronounced “spark”) chemistry is our newest HiFi chemistry that makes sequencing more efficient and expands HiFi sequencing to more sample types and applications. SPRQ requires only 500 ng of native DNA, which opens up even more possibilities for your projects. Plus, SPRQ helps lower genome costs by increasing HiFi yields per SMRT Cell by 33%, allowing you to sequence two human genomes at 20X coverage per SMRT Cell.

And that’s not all—SPRQ also enables deeper multiomic insights into chromatin architecture with Fiber-seq, using on-instrument 5mC and 6mA calling for greater precision in methylation detection.

Search the SDS library to find region-specific SDS in your native language. If you can’t find the SDS you’re looking for, reach out to techsupport@pacb.com.

Extraction from Blood (mammalian and non-mammalian), cultured cell and bacterial samples can be automated on the Thermo Fisher KingFisher and Hamilton NIMBUS Presto instruments using Nanobind HT kits. HT protocols for different sample types and automation systems are listed in this Overview.

On our Technologies page we have a summary of Nanobind manual protocols and on Consumable page an overview of PanDNA and CBB kits protocols.

In this video https://www.pacb.com/videos/extraction-to-revio-system-weve-got-you-covered/, you’ll learn about the extraction of high molecular weight DNA, which is optimal for long-read sequencing, the positive correlation that exists between sample quality and sequencing performance, new whole genome and micro lab prep method kits that will scale with your needs, and finally, our compatibility partners who enable sequencing success, all of which is designed with the goal of achieving customizable end-to-end solutions.

To learn more or to order consumables, visit our Technology and Consumables page for Nanobind, sample prep and library prep solutions.

This video walks you through HiFi sequencing bioinformatics for uncovering germline variation. The talk covers how HiFi reads can provide a better view of genome completeness, an overview of SMRT Link and the GitHub tools that are available for variant calling, information about the PacBio Whole Genome Sequencing variant pipeline, and finally, we take a look at the PacBio compatible partners ecosystem and some helpful additional resources to get you started.

This educational video walks you through the why, what and how of the PacBio Kinnex kits, based on the MAS-Seq method. Specifically, why do you need long reads for RNA sequencing? What are the Kinnex kits? And finally, how do the Kinnex arrays work?

You can find the protocols (procedure & checklists) on the protocols page (filtered view from featured documentation).

24 reaction kit: SMRTbell prep kit 3.0 (102-182-700)

96 reaction kit: HiFi prep kit 96 (103-381-200)

Both kits and protocols use the same enzymatic steps and have qualified automated methods available. Enzyme volumes do differ between protocols/kits, and the HiFi prep kit 96 comes with additional SMRTbell cleanup beads for the automated pipette shearing method and additional fill volume to enable up to 4 automated runs of a minimum of 24 samples.

Yes, the Revio HiFi prep kit 96 (103-382-200) and protocol provides an automated solution to all steps of the workflow going from DNA to a sequence–ready library for HiFi systems. Qualified methods are available on the Hamilton NGS STAR MOA system.

PacBio has developed automation methods for extraction on the Thermo Fisher Kingfisher Apex, Flex, and Duo. PacBio has also developed methods for size selection using SRE, shearing with pipette tips, and bead cleanup on the Hamilton Microlab Prep. You can find all of these methods at the third-party equipment webpage. For methods on any other equipment, please reach out to the support team of the appropriate vendor.