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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. For region-specific safety data sheets (SDS), search the SDS library. For product inserts and more see Other Documentation.

December 12, 2024

Operations guide — Revio system

  • Guide
  • Revio system
  • Sequencing

Description

This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link user guide.
December 10, 2025

Guide & overview — Automated-HiFi-prep-96-and-HiFi-ABC-for-the-Hamilton-NGS-STAR-MOA-system

  • Guide & overview
  • Vega system, Revio system, Sequel II system and Sequel IIe system
  • Library preparation, Automation

Description

This Guide & Overview describes the high throughput automated workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96 on the Hamilton NGS Microlab STAR MOA system.
November 19, 2025

Guide & overview — Short Read Eliminator SRE DNA shearing and cleanup for the Hamilton Microlab Prep system

  • Guide & overview
  • Sequel II system and Sequel IIe system, Revio system, Vega system
  • Library preparation, Automation

Description

This Guide & overview describes the sample, reagent, and consumable preparation for Short Read Eliminator (SRE), DNA shearing, and post-shear cleanup on the Hamilton Microlab Prep (MLP) Liquid Handling system. This Guide & overview is meant to be used with the HiFi prep kit 96, HiFi plex prep kit 96, or SMRTbell® prep kit 3.0 library protocols.
October 29, 2025

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system, Vega system
  • Library preparation

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio HiFi systems.
September 23, 2025

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

  • Procedure & checklist
  • Vega system, Revio system
  • Sample preparation, DNA Shearing, Library preparation

Description

This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems.
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