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Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems.

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

Description

This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems.

Procedure & checklist — Preparing Onso libraries from genomic DNA for short read sequencing

Description

This procedure describes the workflow for constructing Onso libraries from high molecular weight genomic DNA for sequencing on PacBio short-read sequencing systems. This procedure produces PCR-free and/or PCR- amplified libraries for clustering.

Procedure & checklist — Preparing multiplexed AAV SMRTbell libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to prepare multiplexed AAV libraries for sequencing on the Sequel II and Sequel IIe systems. Multiplexing is achieved using SMRTbell barcoded adapters.

Guide & overview — Short Read Eliminator SRE DNA shearing and cleanup for the Hamilton Microlab Prep system

Description

This Guide & overview describes the sample, reagent, and consumable preparation for Short Read Eliminator (SRE), DNA shearing, and post-shear cleanup on the Hamilton Microlab Prep (MLP) Liquid Handling system. This Guide & overview is meant to be used with the HiFi prep kit 96, HiFi plex prep kit 96, or SMRTbell® prep kit 3.0 library protocols.

Procedure & checklist — Extracting HMW DNA from insects using the Nanobind PanDNA kit

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from insects and other arthropods. It is recommended for PacBio HiFi sequencing. This protocol requires the Nanobind PanDNA kit (103-260-000).

Procedure & checklist — Extracting HMW DNA from animal tissue using the Nanobind PanDNA kit

Description

This procedure can be used to extract HMW DNA from animal tissues using the Nanobind PanDNA kit

Procedure & checklist — Extracting HMW DNA from plant nuclei using Nanobind kits

Description

This procedure can be used to extract HMW DNA from plant nuclei.

Procedure & checklist — Isolating nuclei from plant tissue using LN2 disruption

Description

This procedure can be used to isolate nuclei from plant tissues for HMW DNA extraction using the Nanobind plant nuclei kit

Procedure & checklist — Isolating nuclei from plant tissue using TissueRuptor disruption

Description

This procedure can be used to isolate nuclei from plant tissues for HMW DNA extraction using the Nanobind plant nuclei kit

Procedure & checklist — Removing short DNA fragments with the Short Read Eliminator (SRE) kit

Description

This procedure describes the workflow for DNA size selection to remove molecules <10 kb using the SRE kit on high molecular weight (HMW) DNA before shearing and library preparation.

Guide & overview — Automated-HiFi-prep-96-and-HiFi-ABC-for-the-Hamilton-NGS-STAR-MOA-system

Description

This Guide & Overview describes the high throughput automated workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96 on the Hamilton NGS Microlab STAR MOA system.

Procedure & checklist — Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.

Guide & overview — Automated HiFi plex prep 96 for the Hamilton NGS STAR MOA system

Description

This Guide & Overview describes the workflow for PacBio high-throughput semi-automated long read sample prep utilizing the HiFi plex prep 96 kit on the Hamilton NGS Microlab STAR MOA system. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Procedure & checklist — Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio HiFi systems