Featured Documentation

Procedure & checklist — Removing short DNA fragments with the Short Read Eliminator (SRE) kit

Description

This procedure describes the workflow for DNA size selection to remove molecules <10 kb using the SRE kit on high molecular weight (HMW) DNA before shearing and library preparation.

Procedure & checklist — Extracting HMW DNA from cultured adherent cells using Nanobind kits

Description

This procedure can be used to extract HMW DNA from cultured adherent cells.

Procedure & checklist — Extracting HMW DNA from Gram-negative bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-negative bacteria.

Procedure & checklist — Extracting HMW DNA from Gram-positive bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-positive bacteria.

Procedure & checklist — Extracting HMW DNA from nucleated red blood cells using Nanobind kits

Description

This procedure can be used to extract HMW DNA from nucleated red blood cells using Nanobind kits.

Procedure & checklist — Extracting HMW DNA from human whole blood with RBC lysis using the Nanobind PanDNA kit

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from 400 μL of human whole blood with RBC lysis. It is recommended for PacBio® HiFi sequencing. This protocol requires the Nanobind PanDNA kit (103-260-000).

Procedure & checklist — Extracting DNA from cultured suspension cells using Nanobind kits

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from cultured suspension cells. It is recommended for PacBio HiFi sequencing. This protocol requires the Nanobind CBB kit (102-301-900) or the Nanobind PanDNA kit (103-260-000).

Procedure & checklist — Extracting HMW DNA from insects using the Nanobind PanDNA kit

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from insects and other arthropods. It is recommended for PacBio HiFi sequencing. This protocol requires the Nanobind PanDNA kit (103-260-000).

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for cultured bacteria on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from cultured Gram-negative and Gram-positive bacteria using the Hamilton NIMBUS Presto robotic system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for cultured bacteria on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from cultured Gram-negative and Gram-positive bacteria using the Thermo Fisher KingFisher Apex robotic system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for non human mammalian blood NHMB on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of non-human mammalian blood (NHMB) using the Thermo Fisher KingFisher Apex system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for non-human mammalian blood NHMB on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50-300 kb) DNA from 200 μL of non-human mammalian blood (NHMB) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Thermo Fisher KingFisher Apex system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting-HMW DNA from plant nuclei using the Nanobind PanDNA kit

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from plant nuclei. It is recommended for PacBio HiFi sequencing. This protocol requires the Nanobind PanDNA kit (103-260-000).