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Welcome to the PacBio training page. Here, you can search for and download online webinars, e-Modules, and other training documents. If you have any questions about our training materials, please contact us.

December 11, 2025

Technical overview — Kinnex library preparation using Kinnex single-cell RNA kit

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Sample preparation, Library preparation, Binding, Sequencing, Primary Analysis

This technical training presentation provides an overview of Kinnex library preparation using the Kinnex single-cell RNA kit. Sequencing preparation workflow details for Kinnex single-cell RNA libraries as well as example sequencing performance data are also presented.
December 11, 2025

Technical overview — Kinnex library preparation for full-length 16S rRNA sequencing

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system, Vega system
  • Sample preparation, Library preparation, Binding, Sequencing, Primary Analysis

This technical training presentation provides an overview of Kinnex library preparation for full-length 16S rRNA gene sequencing. Sequencing preparation workflow details for Kinnex 16S rRNA libraries as well as example sequencing performance data are also presented.
December 11, 2025

Technical overview — Kinnex library preparation using Kinnex full-length RNA kit

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system, Vega system
  • Sample preparation, Library preparation, Binding, Sequencing, Primary Analysis

This technical training presentation provides an overview of Kinnex library preparation using the Kinnex full-length RNA kit. Sequencing preparation workflow details for Kinnex full-length RNA libraries as well as example sequencing performance data are also presented.
December 11, 2025

Technical overview — Kinnex kits for single-cell RNA and full-length RNA and 16S rRNA sequencing

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system, Vega system
  • Sample preparation, Library preparation, Binding, Sequencing, Primary Analysis

This technical training presentation provides a general overview of Kinnex library preparation using the Kinnex single-cell RNA kit, Kinnex full-length RNA kit and the Kinnex 16S rRNA kit. Sequencing preparation workflow details for Kinnex libraries as well as example sequencing performance data are also presented.
November 19, 2025

Technical overview – Whole genome and metagenome library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Sample preparation, Library preparation, Binding, Sequencing, Primary Analysis

This technical training presentation describes workflow details for constructing whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using SMRTbell prep kit 3.0 (SPK 3.0) for sequencing on PacBio Sequel II, Sequel IIe and Revio systems. Options for multiplexing WGS samples using indexed SMRTbell adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations for de novo assembly, variant detection and shotgun metagenomics applications are also presented.
June 27, 2025

Technical overview — HiFi library preparation using HiFi prep kits for high-throughput sequencing on PacBio long-read systems

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Sample preparation, Library preparation, Binding, Sequencing, Primary Analysis

This technical training presentation provides an overview of HiFi library preparation using HiFi prep kit 96 and HiFi plex prep kit 96. Sequencing preparation workflow details for HiFi prep kit libraries as well as example sequencing performance data are also presented.
February 3, 2025

Technical overview — PureTarget repeat expansion panel library preparation using PureTarget kit

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system, Vega system
  • Sample preparation, Library preparation, Binding, Sequencing, Primary Analysis

This technical training presentation provides an overview of PureTarget library preparation for PureTarget repeat expansion panel sequencing. Sequencing preparation workflow details for PureTarget repeat expansion panel libraries as well as example sequencing performance data are also presented.
June 13, 2023

Technical overview – MAS-Seq library preparation using the MAS-Seq for 10x Single Cell 3’ kit

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Sample preparation, Library preparation, Binding, Sequencing, Primary Analysis

This technical training presentation provides an overview of multiplexed arrays sequencing (MAS-Seq) library preparation using the using the MAS-Seq for 10x Single Cell 3’ kit. Sequencing preparation workflow details, example sequencing performance data, and single-cell MAS-Seq data analysis recommendations are also presented. Step-by-step TSO artifact removal demo video is available.
April 5, 2023

Technical overview — Nanobind HT kits for automated HMW DNA extraction

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Sample preparation, Library preparation

This technical training presentation describes Nanobind HT HMW DNA extraction kits for enabling high-throughput DNA sample preparation automation on four robotic platforms: Hamilton Microlab NIMBUS Presto, Thermo Fisher KingFisher Apex, Flex & Duo Prime systems. Example HiFi sequencing performance data for PacBio SMRTbell libraries constructed from Nanobind HT-extracted DNA are also presented.
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