Training

Training - Ampli-Fi library preparation using PCR for HiFi sequencing on PacBio long-read systems

This technical training presentation provides an overview of PureTarget library preparation for PureTarget repeat expansion panel sequencing. Sequencing preparation workflow details for PureTarget repeat expansion panel libraries as well as example sequencing performance data are also presented.

This technical training presentation provides an overview of key features & benefits of the new Revio system v13.3 + SPRQ chemistry and SMRT Link v25.1 release.

This technical training presentation provides a general overview of Kinnex library preparation using the Kinnex single-cell RNA kit, Kinnex full-length RNA kit and the Kinnex 16S rRNA kit. Sequencing preparation workflow details for Kinnex libraries as well as example sequencing performance data are also presented.

This technical training presentation provides an overview of Kinnex library preparation using the Kinnex single-cell RNA kit. Sequencing preparation workflow details for Kinnex single-cell RNA libraries as well as example sequencing performance data are also presented.

This technical training presentation provides an overview of Kinnex library preparation for full-length 16S rRNA gene sequencing. Sequencing preparation workflow details for Kinnex 16S rRNA libraries as well as example sequencing performance data are also presented.

This technical training presentation provides an overview of Kinnex library preparation using the Kinnex full-length RNA kit. Sequencing preparation workflow details for Kinnex full-length RNA libraries as well as example sequencing performance data are also presented.

High-molecular weight (HMW) DNA sample preparation for PacBio long-read sequencing using Nanobind PanDNA and Short read eliminator (SRE) kits

This technical training presentation describes workflow details for constructing whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using SMRTbell prep kit 3.0 (SPK 3.0) for sequencing on PacBio Sequel II, Sequel IIe and Revio systems. Options for multiplexing WGS samples using indexed SMRTbell adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations for de novo assembly, variant detection and shotgun metagenomics applications are also presented.

This technical training presentation provides an overview of HiFi library preparation using HiFi prep kit 96 and HiFi plex prep kit 96. Sequencing preparation workflow details for HiFi prep kit libraries as well as example sequencing performance data are also presented.

Automated high-throughput high-molecular weight (HMW) DNA extraction for PacBio long-read sequencing using Nanobind HT kits

This technical training presentation provides an overview of multiplexed arrays sequencing (MAS-Seq) library preparation using the using the MAS-Seq for 10x Single Cell 3’ kit. Sequencing preparation workflow details, example sequencing performance data, and single-cell MAS-Seq data analysis recommendations are also presented. Step-by-step TSO artifact removal demo video is available.

This technical training presentation describes Nanobind HT HMW DNA extraction kits for enabling high-throughput DNA sample preparation automation on four robotic platforms: Hamilton Microlab NIMBUS Presto, Thermo Fisher KingFisher Apex, Flex & Duo Prime systems. Example HiFi sequencing performance data for PacBio SMRTbell libraries constructed from Nanobind HT-extracted DNA are also presented.

This technical training presentation provides an overview of multiplexed amplicon library sample preparation using SMRTbell prep kit 3.0 (SPK 3.0) in conjunction with barcoded primers, SMRTbell barcoded adapters or barcoded M13 primers. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations are also presented. Additional guidance for preparing multiplexed amplicon libraries for full-length 16S amplicon sequencing, Human Leukocyte Antigen (HLA) sequencing and HiFi target capture enrichment sequencing (using Twist Bioscience panels) is also summarized.

This technical training presentation provides an overview of multiplexed library preparation for full-viral genome sequencing using SMRTbell prep kit 3.0 (SPK 3.0) and the HiFiViral SARS-CoV-2 Kit for viral genome enrichment. Sequencing preparation workflow details, example sequencing performance data, and HiFiViral SARS-CoV-2 data analysis recommendations are also presented.