Workflow Step: Library preparation
December 2, 2024
Where can I find safety data sheets (SDS)?
Search the SDS library to find region-specific SDS in your native language. If you can’t find the SDS you’re looking for, reach out to techsupport@pacb.com.
August 1, 2024
How can I use HiFi bioinformatics to uncover germline variation?
This video walks you through HiFi sequencing bioinformatics for uncovering germline variation. The talk covers how HiFi reads can provide a better view of genome completeness, an overview of SMRT…
August 1, 2024
Why would you need long reads for RNA sequencing and how do the Kinnex kits work?
This educational video walks you through the why, what and how of the PacBio Kinnex kits, based on the MAS-Seq method. Specifically, why do you need long reads for RNA…
July 12, 2024
Where can I learn about the extraction of high molecular weight DNA, which is optimal for long-read sequencing?
In this video https://www.pacb.com/videos/extraction-to-revio-system-weve-got-you-covered/, you’ll learn about the extraction of high molecular weight DNA, which is optimal for long-read sequencing, the positive correlation that exists between sample quality and sequencing…
May 6, 2024
Does PacBio have a fully automated workflow solution?
Yes, the Revio HiFi prep kit 96 (103-382-200) and protocol provides an automated solution to all steps of the workflow going from DNA to a sequence–ready library for HiFi systems. …
May 6, 2024
Which library prep kit should I use for preparing libraries for Microbial WGS on HiFi systems?
HiFi plex prep kit 96. This kit and protocol offer the most cost efficient and streamlined prep for doing microbial WGS on HiFi systems.
May 6, 2024
Which library prep kit should I use for human, plant, or animal WGS on HiFi sequencing systems?
24 reaction kit: SMRTbell prep kit 3.0 (102-182-700) 96 reaction kit: HiFi prep kit 96 (103-381-200) Both kits and protocols use the same enzymatic steps and have qualified automated methods…
April 24, 2024
For PureTarget, can I use samples that are not human blood and cell line extracted with Nanobind?
The only supported sample type is human blood or cell line extracted with PanDNA or CBB kits. We have been successful internally and at customer sites using other human tissues…